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RAPD Technique, a revolutionary method to detect DNA Polymorphism.

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   HEREDITY HEALTHCARE AND LIFE SCIENCES Contact us: Dr.GopalPurohit Email:  gopal.purohit@hdls.in Contact number:  7381298980 Website: www.heredity.in              Written by : Saptarshi Bhattacharyya The nature of DNA, obtained by different conventions and exposed to different capacity conditions can be decided by contrasting the PCR enhancement profiles. The most normally utilized strategy that can be gotten by practically any research facility is RAPD . Rapid amplification polymorphic DNA (RAPD) is a PCR based procedure for recognizing genetic variations.  It includes the utilization of a single, arbitrary primer in a PCR response, coming about in the enhancement of numerous discrete DNA products.  The strategy was grown freely by two various research centers (Williams et. al., 1990; Welsh and McClelland, 1990) and called RAPD and AP-PCR (Arbitrary prepared PCR) separately. This system recognizes nucleotide sequence polymorphisms in a DNA amplification based measure utilizing just
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The Omicron Variant

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HEREDITY HEALTHCARE AND LIFE SCIENCES Contact us: Dr.GopalPurohit Email:  gopal.purohit@hdls.in Contact number:  7381298980 Website: www.heredity.in             Written by : Saptarshi Bhattacharyya The Omicron variation is a variation of SARS-CoV-2, the infection that causes COVID-19. As of December 2021, it is the most current variation. It was first reported to the World Health Organization (WHO) from South Africa on 24 November 2021. On 26 November 2021, the WHO assigned it as a variation of concern and named it "Omicron", the fifteenth letter in the Greek letters in order. The variation has a bizarrely enormous number of changes, a few of which are novel and countless which influence the spike protein focused on by most COVID-19 antibodies at the hour of the revelation of the Omicron variation. This degree of variety has prompted concerns in regards to its contagiousness, invulnerable framework avoidance, and immunization obstruction, in spite of starting reports demonstr
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CFTR Gene Mutation Analysis

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  HEREDITY HEALTHCARE AND LIFE SCIENCES Contact us: Dr.GopalPurohit Email: gopal.purohit@hdls.in Contact number: 7381298980 Website: www.heredity.in     Written by: Sweta Yadav Introduction: Cystic fibrosis (CF)  is an inherited disease that affects mainly the lungs, pancreas, and sweat glands. It leads to the production of thick, sticky mucus and can cause recurrent respiratory infections and impaired function of the pancreas. The CF gene mutation panel detects the common mutations in the cystic fibrosis transmembrane conductance regulator ( CFTR ) gene on chromosome 7 to screen for or diagnose CF or to identify carriers of the disease. Cystic fibrosis is caused by a mutation in each of the two copies of the  CFTR  gene (one copy from each parent). Both copies (alleles) of this gene pair must be abnormal to cause CF. If only one copy is mutated, the individual is a CF carrier. Carriers do not generally have any CF symptoms, but they can pass their abnormal
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Next Generation Sequencing (NGS)

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  HEREDITY HEALTHCARE AND LIFE SCIENCES Contact us: Dr.Gopal Purohit Email: gopal.purohit@hdls.in Contact number: 7381298980 Website: www.heredity.in     Written by: Sweta Yadav Introduction: ·          Next Generation Sequencing (NGS) is a powerful platform that has enabled the sequencing of thousands to millions of  DNA  molecules simultaneously. ·          Next-generation sequencing (NGS), also known as high-throughput sequencing, is the catch-all term used to describe a number of different modern sequencing technologies. ·          The high demand for low-cost sequencing has driven the development of high-throughput sequencing which produce thousands or millions of sequences at once. ·          They are intended to lower the cost of DNA sequencing beyond what is possible with standard dye-terminator methods. ·          Thus, these recent technologies allow us to sequence DNA and RNA much more quickly and cheaply than the previously used Sanger seque
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IMMUNO-ONCOLOGY

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            HEREDITY HEALTHCARE AND LIFE                                 SCIENCES                             Contact us:                         Dr.Gopal Purohit                  Email: gopal.purohit@hdls.in                 Contact number: 7381298980                    Website: www.heredity.in            Written by: Sweta Yadav Introduction: Normally, our immune system is able to destroy cancer cells in our body, however sometimes cancer cells can adapt and mutate, effectively hiding from our immune system. This is when tumours can develop and become a threat to our health. Immuno-oncology involves mobilising lymphocytes to recognize and eliminate cancer cells using the body’s immune system. In theory, this is already much better for patients than the current treatments for cancer, which mainly are surgery, chemotherapy and radiation. These treatments likely cause as much harm to patients  as they do good, either leaving deadly cancer cells untreated, or killing off too many healthy c
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ILLUMINA SEQUENCING - THE BASIS OF NEXT GENERATION SEQUENCING :-

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  HEREDITY HEALTHCARE AND LIFE SCIENCES Contact us: Dr.Gopal Purohit Email:gopal.purohit@hdls.in Contact number:7381298980 Website:  www.heredity.in     Written by:  SAPTARSHI BHATTACHARYYA In principle, the concept behind NGS technology is similar to CE sequencing. DNA polymerase catalyzes the incorporation of fluorescently labeled deoxyribonucleotide triphosphates (dNTPs) into a DNA template strand during sequential cycles ofDNA synthesis. During each cycle, at the point of incorporation, the nucleotides are identified by fluorophore excitation. Thecritical difference is that, instead of sequencing a single DNA fragment, NGS extends this process across millions of fragmentsin a massively parallel fashion. More than 90% of the world's sequencing data are generated by Illumina sequencing bysynthesis (SBS) chemistry . Illumina NGS workflows include four basic steps: 1. Library Preparation —The sequencing library is prepared by random fragmentation of the DNA or cDNA sample, follow
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