Next Generation Sequencing (NGS)

 

HEREDITY HEALTHCARE AND LIFE SCIENCES


Contact us:

Dr.Gopal Purohit

Email: gopal.purohit@hdls.in

Contact number: 7381298980

Website:www.heredity.in

 

 Written by: Sweta Yadav





Introduction:

·         Next Generation Sequencing (NGS) is a powerful platform that has enabled the sequencing of thousands to millions of DNA molecules simultaneously.

·         Next-generation sequencing (NGS), also known as high-throughput sequencing, is the catch-all term used to describe a number of different modern sequencing technologies.

·         The high demand for low-cost sequencing has driven the development of high-throughput sequencing which produce thousands or millions of sequences at once.

·         They are intended to lower the cost of DNA sequencing beyond what is possible with standard dye-terminator methods.

·         Thus, these recent technologies allow us to sequence DNA and RNA much more quickly and cheaply than the previously used Sanger sequencing, and as such have revolutionized the study of genomics and molecular biology.

 

Classified into different generations, NGS has led to overcome the limitations of conventional DNA sequencing methods and has found usage in a wide range of molecular biology applications.

 

It is classified into different generations that include:

First Generation

·         Sanger Sequencing

 

Second Generation Sequencing

·         Pyrosequencing

·         Sequencing by Reversible Terminator Chemistry

·         Sequencing by Ligation

 

 Third Generation Sequencing

·         Single Molecule Fluorescent Sequencing

·         Single Molecule Real Time Sequencing

·         Semiconductor Sequencing

·         Nanopore Sequencing

 

Fourth Generation Sequencing

·           Aims conducting genomic analysis directly in the cell.

 

 

Important Next Generation Sequencing Techniques:

·  ·Lynx therapeutics’ massively parallel signature sequencing (MPSS)

·      ·    Polony sequencing

·     ·    Pyro sequenceing

·     · Illumina (Solexa) sequencing

·     ·    SOLiD sequencing

·    ·     DNA Nanoball sequencing

·    ·     Helioscope single molecule sequencing

·    · Single molecule SMRT sequencing

· ·Single molecule real time (RNAP) sequencing

 

 

References:

1.1. http://library.umac.mo/ebooks/b2805039pdf

2. 2 .David Hames and Nigel Hooper (2005). Biochemistry. Third ed. Taylor & Francis Group: New York.

3. 3. http://www.aun.edu.eg/molecular_biology/Proceeding_Dec2011/DNA%20sequencing.pdf

4.4.https://ab.inf.uni-tuebingen.de/teaching/ws09/bioinformatics-i/10-sequencing.pdf

5. 5.  http://www.pathologyoutlines.com/topic/molecularpathdnaseqmaxam.html

 

 

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