heredityinnovation

  HEREDITY HEALTHCARE AND LIFE SCIENCES Contact us: Dr.Gopal Purohit Email: gopal.purohit@hdls.in Contact number: 7381298980 Website: www.heredity.in     Written by: Sweta Yadav Introduction: ·          Next Generation Sequencing (NGS) is a powerful platform that has enabled the sequencing of thousands to millions of  DNA  molecules simultaneously. ·          Next-generation sequencing (NGS), also known as high-throughput sequencing, is the catch-all term used to describe a number of different modern sequencing technologies. ·          The high demand for low-cost sequencing has driven the development of high-throughput sequencing which produce thousands or millions of sequences at once. ·          They are intended to lower the cost of DNA sequencing beyond what is possible with standard dye-terminator methods. ·          Thus, these recent technologies allow us to sequence DNA and RNA much more quickly and cheaply than the previously used Sanger seque

            HEREDITY HEALTHCARE AND LIFE                                 SCIENCES                             Contact us:                         Dr.Gopal Purohit                  Email: gopal.purohit@hdls.in                 Contact number: 7381298980                    Website: www.heredity.in            Written by: Sweta Yadav Introduction: Normally, our immune system is able to destroy cancer cells in our body, however sometimes cancer cells can adapt and mutate, effectively hiding from our immune system. This is when tumours can develop and become a threat to our health. Immuno-oncology involves mobilising lymphocytes to recognize and eliminate cancer cells using the body’s immune system. In theory, this is already much better for patients than the current treatments for cancer, which mainly are surgery, chemotherapy and radiation. These treatments likely cause as much harm to patients  as they do good, either leaving deadly cancer cells untreated, or killing off too many healthy c

  HEREDITY HEALTHCARE AND LIFE SCIENCES Contact us: Dr.Gopal Purohit Email:gopal.purohit@hdls.in Contact number:7381298980 Website:  www.heredity.in     Written by:  SAPTARSHI BHATTACHARYYA In principle, the concept behind NGS technology is similar to CE sequencing. DNA polymerase catalyzes the incorporation of fluorescently labeled deoxyribonucleotide triphosphates (dNTPs) into a DNA template strand during sequential cycles ofDNA synthesis. During each cycle, at the point of incorporation, the nucleotides are identified by fluorophore excitation. Thecritical difference is that, instead of sequencing a single DNA fragment, NGS extends this process across millions of fragmentsin a massively parallel fashion. More than 90% of the world's sequencing data are generated by Illumina sequencing bysynthesis (SBS) chemistry . Illumina NGS workflows include four basic steps: 1. Library Preparation —The sequencing library is prepared by random fragmentation of the DNA or cDNA sample, follow